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HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY (HILIC)
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The ZIC®-HILIC and ZIC®-pHilic columns are the ideal choice for separation of polar and hydrophilic compounds as this column is truly orthogonal to reversed phase chromatography (RPLC) columns. Analytes such as amino acids, peptides, carbohydrates, plant extracts and various other polar compounds that usually have little or no retention and may be affected by wettability problems in RPLC, generally have strong retention on the ZIC®-HILIC and ZIC®-pHilic column. |
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| The zwitterionic ZIC®-HILIC stationary phase is attached to porous silica and is available in columns from capillary to preparative dimensions. The separation is achieved by a hydrophilic partitioning mechanism superimposed on weak electrostatic interactions. Typical eluents contain a high content of an organic solvent (e.g., acetonitrile) mixed with buffer, i.e., comparable to RPLC eluents, however, water being the strong solvent. Also, the low content of water in the eluents enables higher detection sensitivity in many LC/MS applications. |
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ZIC®-pHILIC columns have a zwitterionic stationary phase on porous polymer particles and the separation is achieved by a hydrophilic interaction mechanism superimposed on weak electrostatic interactions.
Due to the polymeric support particle, the column can be operated in a broad pH range, which can be used to improve the retention and selectivity for many compounds. Also, the detection sensitivity in LC/MS applications might benefit from the use of eluents in the alkaline pH range.
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For the seperation of:
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For the seperation of:
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| Aminoacid, Peptide, Nucleotide, Amine, Phenole, Acdis, Carbohydrates, Glucuronide and Glycuronide Compounds, Phosphoryated Compounds, Saphonine .....
all molecules that elute close to the hold-up time RP Phase or that use ion-interaction reagent
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Seperation in a broad pH range |
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| Base-Material: |
Silica |
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| Functional Group: |
Sulfobetaine |
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| Pore Size: |
100 Å, 200 Å |
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| Particle Size: |
3.5, 5, 10, 16, 50 µm |
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| pH-Stability |
3 - 8 |
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| Base-Material: |
Polymer |
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| Functional Group: |
Sulfobetaine |
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| Pore Size: |
200 Å |
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| Particle Size |
5 µm |
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| pH-Stability |
1 - 12 |
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